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Hua Hsi I Ko Ta Hsueh Hsueh Pao 1996 Jun;27(2):209-12

[Subcellular location of blood group substances ABH using transmission electron microscopical immunocytochemistry technique].

[Article in Chinese]

Yang G, Li R, Wu M, Li Q, Yi X

Department of Electron Microscopy, Chengdu.

The subcellular location of blood group substances ABH was studied by using transmission electron microscopical immunocytochemistry. The cells were the epithelial cells of both mucous membranes and glands of normal human stomach, duodenum and transverse colon. Both cellular ultrastructure and antigens should be well preserved in transmission electron microscopical immunocytochemistry. In this paper, various fixatives, embedding media and immunostaining methods were investigated and compared. The results indicate that the solution containing 2% paraformaldehyde and 0.2% glutaraldehyde (2PG) is the best fixative and Epon 812 medium is the best embedding medium for preserving ABH antigens and protecting cellular ultrastructure. Post-embedding immunogold staining technique is superior to pre-embedding immunoperoxidase staining.

PMID: 9389046, UI: 98050429

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Hua Hsi I Ko Ta Hsueh Hsueh Pao 1996 Jun;27(2):146-50

[Genetic polymorphism of antithrombin III in five Chinese populations].

[Article in Chinese]

Gou Q, Wu J, Hou Y, Xu Y, Jin Z, Wu M

Department of Forensic Biology, Chengdu.

The genetic polymorphisms of antithrombin III (AT III) in 5 Chinese populations were studied by isoelectric focusing on polyacrylamide gels followed by immunoblotting. The products of two alleles at the antithrombin III locus together with 1 AT III anodic variant and 3 cathode variants were observed. The results revealed that the predominant allele of AT III blood group system in Chinese populations was AT III *1, with frequencies between 0.9773-0.9850. Frequencies of AT III *2 ranged from 0.0091 to 0.0169. These results indicate that the polymorphic information content of AT III blood group system in Chinese populations is limited.

PMID: 9389029, UI: 98050412

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Hua Hsi I Ko Ta Hsueh Hsueh Pao 1996 Mar;27(1):21-5

[Simultaneous phenotyping of proteins with various pHs by complex IEF and its application in forensic haemogenetics].

[Article in Chinese]

Hou Y, Gou Q, Wu M

We have developed a new technique of complex isoelectric focusing (IEF) by which various proteins with different pHs can be detected out in polyacrylamide gels containing ampholytes with different pH ranges at the same time. The success of phenotyping 12 human proteins by this technique proved it was reliable and efficient. The cumulative Epp and the cumulative Dp reached 0.9 and 0.9999 respectively. It provides a new approach to analysis of genetic markers in the field of forensic haemogenetics.

PMID: 9208614, UI: 97352334

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Hua Hsi I Ko Ta Hsueh Hsueh Pao 1995 Dec;26(4):424-6

[Genetic polymorphism of properdin factor B in the Han population in Chengdu, China].

[Article in Chinese]

Chen G, Gou Q, Hou Y, Wu M

The genetic polymorphism of properdin factor B(Bf) was first investigated in the Han population in Chengdu by isoelectrofocusing and immunofixation. The results revealed BfSS (129 cases), BfFS (48 cases) and BfFF (9 cases). No Bf variants were noted in this study. The allele frequencies were: Bf * S 0.8226, Bf * F 0.1774.

PMID: 8732066, UI: 96331974

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Hua Hsi I Ko Ta Hsueh Hsueh Pao 1995 Sep;26(3):311-4

[Effects of some physical and chemical factors on both human hair DNA and results of sex determination by PCR].

[Article in Chinese]

Sun G, Xu Y, Wu M

The present study aims at observation of the effects of some physical and chemical factors on both human hair DNA and the results of sex determination by PCR. After the hairs were treated with varying pH solutions, the quantity of the DNA extracted was decreased. High-molecular-weight (HMW) DNA was determined in the hairs treated with pH 1, 3, 5 and 7 solutions for 2 hours, but no DNA was demonstrated by agarose gel electrophoresis in the hairs treated with pH 1 and 3 solution for 24 hours, and the quantity of DNA was decreased in the hairs treated with pH 5 for 24 hours. The quantity of DNA was decreased in the hairs treated with pH 9 for 2 hours and no DNA was demonstrated for 24 hours. No DNA was found in the hairs treated with pH 11 for 2 or 24 hours. No changes of DNA were observed in hairs treated with 75% ethanol or methanol for 2 or 24 hours and exposed to UV light for 24 hours. No DNA but RNA was observed in the hairs treated with 10% formalin (pH 7 or pH 5) for 2 or 24 hours. The quantity of DNA was decreased obviously in the hairs treated in 100 degrees C water for 5 or 10 minutes. The quantity of DNA was not decreased in the hairs heated at 50 degrees C for 24 hours, and only little decrease was noted at 100 degrees C for 24 hours. Both fragments of PCR products were obtained in most hairs except the hairs treated with pH 11 solution for 24 hours. Although no DNA was observed in some treated hairs, specific bands were detected after PCR. PCR is a very sensitive and specific technique of DNA amplification, which can be used for sex determination in forensic medicine.

PMID: 8586399, UI: 96147950

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Hua Hsi I Ko Ta Hsueh Hsueh Pao 1995 Mar;26(1):23-8

[Changes of ABH substances amount in human tissues after treatment with physico-chemical factors].

[Article in Chinese]

Li R, Zhang L, Wu M

The forensic samples for identify testing are always affected by the environmental or the physico-chemical influence. The aim of the present study is to study whether the physico-chemical or biological factors affect the quantity of ABH substances in human tissues or not. The quantity of the ABH substances was determined by immunohistochemical ABC method. The tissues were treated by a series of methods, including immersing in water, treatment with heat, acid, alkali as well as putrefaction and mold growing. The results revealed that although the ABH substances slightly decreased in some tissues pretreated, no effect of ABO typing was observed. The correct ABO typing was performed with tissues under the following condition: the tongue and skin immersed in water for 21 days; the tongue treated with heat (100 degrees C) for 40 min; the tongue and skin treated with 0.2mol/L HCl and 0.2 mol/L NaOH for 48 h and the skin treated with 12mol/L HCl and 12mol/L NaOH for 30 min. The ABO typing of pancreas undergoing putrefaction and mold growing 21 days was carried out correctly. It is concluded that the ABC immunohistochemical technique is a good method for ABO typing of human tissues.

PMID: 7657332, UI: 95386176

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Hua Hsi I Ko Ta Hsueh Hsueh Pao 1994 Dec;25(4):398-401

[Fast detection of Alu DNA in human tissues by Alu-PCR].

[Article in Chinese]

Sun G, Chen L, Wu M

This paper reports a direct PCR method for the detection of Alu DNA in 18 different kinds of human tissue using primers of Alu 9.1 and Alu 9.2 without DNA extraction. The results showed that besides fresh tissues, some formalin fixed paraffin embedded tissues can be correctly analyzed with PCR. Different amounts of PCR products were obtained from different types of tissues. The duration of fixation in formalin is an important factor exerting an influence on the quantity of the high molecular weight DNA and the results of PCR. No obvious influence of the duration of storage of paraffin embedded tissues on the PCR results was observed. This method is quite simple, sensitive and rapid to perform. It can be performed under a condition of avoiding contamination of DNA and DNase as well as the detrimental phenol and chloroform.

PMID: 7744381, UI: 95263039

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Hua Hsi I Ko Ta Hsueh Hsueh Pao 1994 Dec;25(4):375-9

[Distribution of ABH substances in normal secretor human tissue cells by avidin-biotin complex method].

[Article in Chinese]

Li R, Zhang L, Wu M

The distribution and location of ABH substances in 54 various kinds of normal secretor human tissue cell of known ABO type were studied by Avidin-Biotin complex (ABC) method. It was firstly demonstrated that 18 different kinds of tissue cells contained ABH substances, which were as follows: neuron and nerve fiber, astrocyte, oligodendrocyte, ependyma epithelial cells, epithelial cells of soft meninges, epithelial cells of parotid gland duct, taste bud cells of tongue, acinar and epithelial cells of duct of buccal gland, epithelial cells of rectal mucous membrane gland, regenerated liver cells of hepatocirrhosis, basal cells of stratified aquamous epithelium of epiglottis, spermiogenesis and spermatozoa of seminiferous tubules, epithelial cells of tubuli reti, rete testis and tubuli epididymis, both primary and secondary follicles of ovary and decidua cells of endometrium. Some new phenomena were observed as follows: ABH substances in chief cells more than those in parietal cells of gastric gland, locally distributed ABH positive cells in stratum spinosum of stratified squamous epithelium of skin, segmentally distributed ABH positive cells in pseudostratified ciliated columanal epithelium of epiglottis. The ABH substances were located at the infranuclear and/or supranuclear region and/or brush border of the epithelial cells of submaxillary gland, mosaic distributed ABH substances were found in the epithelia of both sweat and submaxillary glandular ducts. A few controversies about distribution of ABH substances were solved. The relationship between H and A, B substances, origin of ABH substances, source of ABH substances in body fluids. Production of ABH substances and cell differentiation, mosaicism and inconsistency of ABH distribution are discussed and compared with other reports.

PMID: 7744376, UI: 95263034

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Hua Hsi I Ko Ta Hsueh Hsueh Pao 1994 Sep;25(3):253-8

[Influence exerted by environmental and physicochemical factors on the results of sex identification of human dental pulp by polymerase chain reaction].

[Article in Chinese]

Chen L, Sun G, Wu M

Sex identification of human tooth is an important aspect of individual identification in forensic science practice. This study was designed to determine the effects of environmental and physicochemical factors (EPF) on the deoxyribonucleic acid (DNA) from dental pulp and PCR result. Extracted teeth were subjected to the following treatment varying pH (2, 7, 10) solution; 10% formalin, 75% ethanol; different temperature (-20 degrees C, 4 degrees C, 25 degrees C, 37 degrees C, boiling, burning); humidity (20%, 66%, water submergence); burying the teeth outdoors; and aging (teeth stored at room temperature for 1.5, 3.5 and 8 years). DNA was extracted from the tooth followed by agarose gel electrophoresis for the purpose of DNA assay and quantity determination. Amplification was carried out using a pairs of primer Y1.1 and Y1.2. The results of the study showed that, 154 bp fragment was obtained in all male teeth, whereas no 154 bp fragment was found in a female fresh tooth. 154 bp fragment bands were not observed in case of teeth subjected to burial outdoors for 10 weeks, submergence in water for 10 weeks, soak in pH 2 solution for 10 weeks, and combustion for 10 minutes; only faint bands were observed in case of teeth subjected to 66% humidity and pH 10 solution for 10 weeks and combustion for 5 minutes; obvious bands were observed in the rest cases. In addition, the quality of DNA from dental pulp affected PCR amplification result. The integrity of DNA isolated was categorized as being high-molecular-weight (HMW), HMW with partial degraded DNA, completely degraded DNA, completely degraded and not detected.

PMID: 7896239, UI: 95203839

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Hua Hsi I Ko Ta Hsueh Hsueh Pao 1994 Jun;25(2):142-4

[Confirmative test of human semen stain and mixed stain by immunohistochemical method using anti-human sperm monoclonal antibodies].

[Article in Chinese]

Ou J, Li Y, Wu M

The stain of the human semen, the mixture of the human semen and vaginal secretion as well as the semen of the bull, goat, pig, dog, rabbit, rat and mouse was examined by the immunohistochemical method using anti-human sperm monoclonal antibodies (SMAB). The result revealed that the SMAB E10 could make a distinction between the human semen, the mixture of the human semen and vaginal secretion, and the seven kinds of animal semen. It is concluded that this method can be used for the species identification of the semen and the mixed stain under the general condition.

PMID: 7528713, UI: 95104809

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Hua Hsi I Ko Ta Hsueh Hsueh Pao 1994 Mar;25(1):105-7

[Application of ORM1 phenotyping in forensic science].

[Article in Chinese]

Tan M, Gou Q, Hou Y, Wu M

ORM1 is an important genetic marker for both parentage testing and personal identification in forensic science. We have examined the ORM1 phenotypes in the human serum and dried bloodstains using the ULPAGIF method. ORM1 phenotypes were successfully demonstrated from dried bloodstains stored at 4 degrees C and room temperature for up to twenty-four weeks. In bloodstains stored at 37 degrees C, the 100% of ORM1 phenotyping was no longer possible after 12 weeks. We examined 9 bloodstains stored at room temperature for one, two and three years, respectively. For the one-year-old bloodstains, all of the samples could be phenotyped correctly, for the two-year-old bloodstains 8 out of the 9 and for the three-year-old bloodstains 7 out of the 9 samples could be phenotyped correctly. The results have indicated that ORM1 is relatively stable, for it is tolerable toward high temperature and long duration. It was suggested that the bloodstains should be ultrasonicated before ULPAGIF, because the ultrasonication could elevate the detectable rate of ORM1 phenotypes from bloodstains. ORM1 phenotypes was performed in five disputed parentage cases. One of the alleged fathers was excluded.

PMID: 8070760, UI: 94350313

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Hua Hsi I Ko Ta Hsueh Hsueh Pao 1993 Dec;24(4):373-5

[The influence of prostate-specific antigen p30 on human fertility].

[Article in Chinese]

Gou Q, Hou Y, Zhang N, Wu M, Wang D, Yang S

The influence of prostate-specific antigen p30 on human fertility was studied with purified p30, domestic anti-p30 monoclonal and polyclonal antibodies. The results showed that the anti-p30 antibody presented in human serum had no negative effect upon human fertility and the anti-p30 antibody produced by heteroimmunization did not interfere with the process of human fertility in vitro. All these imply that it is unsuitable to recommend p30 as a contraceptive vaccine antigen.

PMID: 7512068, UI: 94200759

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Hua Hsi I Ko Ta Hsueh Hsueh Pao 1993 Sep;24(3):286-9

[Isolation and purification of alpha -1 acid glycoprotein from human sera].

[Article in Chinese]

Hou Y, Gou Q, Wu M

Alpha-1 acid glycoprotein, also named orosomucoid (ORM), is a serum protein with genetic polymorphism. This paper describes a two-step procedure for purifying ORM from human sera. The procedure consists of both anion exchange chromatography on a DEAE-Sephadex-A50 column and affinity chromatography on a Reactive Blue 2-Sepharose CL-6B column. The purified ORM identified by polyacrylamide gel electrophoresis, SDS-polyacrylamide gradient gel electrophoresis, immunoelectrophoresis and immunodiffusion was homogeneous and reacted specifically with a commercial anti-ORM serum (Sigma). The molecular weight of the purified ORM was about 41 kd.

PMID: 8288200, UI: 94116962

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Hua Hsi I Ko Ta Hsueh Hsueh Pao 1993 Sep;24(3):283-5

[Subtyping of PiM in human serum and semen by modified isoelectric focusing].

[Article in Chinese]

Gou Q, Hou Y, Xu Y, Wu M

An isoelectric focusing of Pi pretreated with neuraminidase is reported. Subtyping of PiM in human semen was successfully carried out for the first time. This has provided a new approach for individual identification in sexual crime.

PMID: 8288199, UI: 94116961

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Hua Hsi I Ko Ta Hsueh Hsueh Pao 1993 Sep;24(3):281-2

[Subtyping of GC globulin in human semen stain].

[Article in Chinese]

Jin Z, Wu M

The group-specific component (GC) is an alpha 2 globulin with high genetic polymorphism in serum. There is also a little amount of GC in human semen. Pflug and Potsch-Schneider independently carried out GC subtyping of human semen stain with immobilized pH gradient gel isoelectric focusing (IPGIF) followed by ELISA in 1988. We report a new method there to subtype GC in human semen stains. First, the GC globulin was separated by polyacrylamide gel isoelectric focusing with pH 4.2-4.9 Pharmalyte. Then, it was immunoblotted to cellulose acetate membranes. Finally, the protein bands were shown by ELISA using horseradish peroxidase-linked secondary antibody system. All the 17 semen stains of volunteers were subtyped successfully. This method is easy to learn and convenient for applying in case work.

PMID: 8288198, UI: 94116960

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Hua Hsi I Ko Ta Hsueh Hsueh Pao 1992 Sep;23(3):248-50

[Human blood stain identification using DNA amplification technique].

[Article in Chinese]

Wu M, Sun G

DNA was extracted from human's and 15 different species of mammals' dried blood stains as well as from camel hair roots. DNA amplification was carried out using primers of Alu 9.1 and Alu 9.2. The results have indicated that this method can be used for the identification of human's dried blood stain under general condition.

PMID: 1298710, UI: 93224106

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Hua Hsi I Ko Ta Hsueh Hsueh Pao 1992 Mar;23(1):58-60

[Detection of G2m(n) factor in bloodstains using ELISA inhibition test].

[Article in Chinese]

Gou Q, Hou Y, Wu M

For the purpose of detecting G2m(n) factor in human bloodstains, an ELISA inhibition test using mouse antihuman G2m(n) monoclonal antibody was established. The results revealed that the correctness rate for G2m(n) factor detection was 100%. The minimal amount of bloodstain required for detecting G2m(n) factor was 0.25 cm x 0.125 cm. This approach provided a new method for individual identification of human bloodstains.

PMID: 1398627, UI: 93013740

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Hua Hsi I Ko Ta Hsueh Hsueh Pao 1991 Sep;22(4):348-51

[Genetic polymorphism of alpha 2HS-glycoprotein in the Han population in Chengdu].

[Article in Chinese]

Hou Y, Gou Q, Wu M

The distribution of alpha 2HS-glycoprotein (AHSG) phenotype frequencies in the Han population in Chengdu was studied using polyacrylamide gel isoelectric focusing followed by immunofixation with rabbit anti-human AHSG serum. Two hundred eighty-six serum samples collected at random from unrelated individuals were phenotyped for AHSG. The distribution of AHSG phenotype frequencies was found to be AHSG 1 = 47.20%, AHSG 2 = 8.04% and AHSG 2 - 1 = 44.76%. The observed numbers agreed well with the expected numbers calculated on the basis of the Hardy-Weinberg equilibrium. The allele frequencies were estimated to be AHSG.1 = 0.6958 and AHSG.2 = 0.3042. The discrimination probability of AHSG is 0.5704 and the exclusion probability of parentage 0.1669.

PMID: 1814809, UI: 92267493

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Hua Hsi I Ko Ta Hsueh Hsueh Pao 1990 Sep;21(3):274-6

[The measurement of p30 level in the normal human seminal plasma by the Beckman immunochemistry system using anti-30 serum].

[Article in Chinese]

Zhou L, Hou Y, Wu M

This paper is the first report about the measurement of p30 level in the normal human seminal plasma by the Beckman immunochemistry system (ICS) using anti-p30 serum. The p30 levels of 108 samples of normal human seminal plasma were measured. The range of p30 level was 0.2996-4.3913 mg/ml. The square root transformation statistical analysis indicated that the coefficient of skewness was 0.0237 the coefficient of kurtosis was -0.8854, the p30 level in normal human seminal plasma fitted the square root normal distribution, the mean was 1.6236 mg/ml and the standard deviation was 0.1641 mg/ml.

PMID: 2093064, UI: 91231305

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Hua Hsi I Ko Ta Hsueh Hsueh Pao 1990 Sep;21(3):271-3

[Human sex identification in dried bloodstains by analysis of amplified DNA sequences].

[Article in Chinese]

Sun G, Wu M

This paper describes a technique of DNA amplification in vitro and its application on sex identification of human dried bloodstains. Dried bloodstains (8 males and 8 females) were prepared on filter paper, and then DNA was extracted. Target DNA sequences (Y-3.4kb repeat and Alu-repeat) were amplified by polymerase chain reaction (PCR) with two pairs of primers (Y1.1, Y1.2 and Alu9.1, Alu9.2). Amplification products were obtained in 8 male's bloodstains with the primers Y1.1 and Y1.2, but not in 8 female's bloodstains; and in 4 male's and 4 female's bloodstains with Alu9.1 and Alu9.2.

PMID: 2093063, UI: 91231304

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Hua Hsi I Ko Ta Hsueh Hsueh Pao 1990 Jun;21(2):121-4

[Physico-chemical characterization of human seminal plasma specific antigen p30].

[Article in Chinese]

Hou Y, Wu M

Human seminal plasma specific antigen p30, which has been characterized by other authors under the name of prostate-specific antigen or gamma-seminoprotein, has been purified. This communication describes the physico-chemical characterization of p30 demonstrated by various SDS-polyacrylamide gel electrophoresis and isoelectrofocusing as well as amino acid analysis. The results showed that the molecular weight of p30 was 33 kd and its isoelectric point was around pH 6.9. Its amino acid composition was similar to that of prostate-specific antigen. It is concluded that p30 purified by our laboratory and prostate-specific antigen named by Wang are the identical one of isomers.

PMID: 1697278, UI: 90361266

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Hua Hsi I Ko Ta Hsueh Hsueh Pao 1990 Mar;21(1):12-6

[Establishment of hybridoma cell lines producing monoclonal antibodies against-p30 antigen].

[Article in Chinese]

Li Y, Wu M, Zhang L, Zhao Q, Zeng Y

BALB/c mice were immunized with the purified p30 antigen. SP2/0 myeloma cells and immune spleen cells were fused with 50% PEG (Sigma, MW 3,350-4,000). The cell fusion rate was 93.95%, and the antibody producing rate 21.01%. The technique of limiting dilution was used for cloning of the hybridoma cells. Two hybridoma cell lines E8 and G1 secreting McAb against p30 antigen were obtained. The number of chromosome of both E8 and G1 cell lines was 98.7 +/- 6.54 and 97.7 +/- 7.77, respectively. Results of the PAGE of the ascites generated by the hybridoma cell lines E8 and G1 showed a thick protein band at the gamma-region which was absent from the ascites generated by the SP 2/0 myeloma cells. The immunoglobulin of the McAb E8 and G1 belonged to IgG2 subclass. The results of the immunohistochemical method using the horseradish peroxidase conjugated antibody showed that both E8 and G1 McAb only reacted with epithelial cells of the normal human prostatic glands and their ducts, but did not cross-react with other thirty-four different kinds of normal human tissues. The results of inhibiting ELISA test showed that both E8 and G1 McAb were only inhibited by the human seminal plasma and p30, weakly inhibited by the adult male's urine, but were not inhibited by other eight different kinds of human body fluids and secretions as well as semen from eight different species of animals. It was concluded that McAb of E8 and G1 were organ and species specific.

PMID: 2365336, UI: 90306977

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Hua Hsi I Ko Ta Hsueh Hsueh Pao 1989 Sep;20(3):321-3

[Subtyping of Pi in human bloodstains by isoelectric focusing].

[Article in Chinese]

Cao ZM, Wu MY

Subtyping of Pi (alpha 1-AT) in 90 human bloodstains stored under different conditions was carried out by using ULPAGIF. The detectable time of Pi subtypes of all bloodstains kept at room temperature (12-20 degrees C), 4 degrees C and-15 degrees C was 10 days, 4 weeks and 4 weeks respectively. Beyond this time limit, the detectable rate decreased gradually. The minimal detectable quantity, of fresh bloodstains was 4 microliters. The factors influencing the detection of Pi subtypes in bloodstains were discussed.

PMID: 2625341, UI: 90169935

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Hua Hsi I Ko Ta Hsueh Hsueh Pao 1989 Sep;20(3):317-21

[Study on application of Tf subtyping in forensic medicine].

[Article in Chinese]

Tan M, Wu MY

Tf is an important genetic marker for both parentage testing and personal identification in forensic medicine. It is valuable in anthropology, genetics and clinical medicine. We examined the Tf subtypes in the human serum and dried bloodstain using the ULPAGIF method. Tf subtyping was performed in four disputed parentage cases. One of the alleged fathers was excluded. Tf subtypes were successfully demonstrated from dried bloodstains kept at 4 degrees C for up to four weeks. In bloodstains kept at room temperature and 37 degrees C, the 100% of Tf subtyping was no longer possible after two weeks (or one day). It was suggested that the bloodstains should be examined no longer than seven weeks if the bloodstains were kept at room temperature; or otherwise they should be stored below 4 degrees C as soon as they are collected in forensic practice.

PMID: 2625340, UI: 90169934

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Hua Hsi I Ko Ta Hsueh Hsueh Pao 1989 Jun;20(2):144-6

[Identification of human semen stains using anti-p30 serum].

[Article in Chinese]

Hou YP, Wu MY

The present communication describes the identification of human semen stains using immunodiffusion test with anti-p30 serum. In order to evaluate objectively the value of the immunodiffusion test for the identification. Random sampling and blind study were used. The results indicated that the immunodiffusion test was more accurate than sperm detection. It was proved that p30 was a useful human semen marker especially in semen stains of azoospermia and vaseligation. The vaginal secretion and saliva in mixed stains did not interfere with the result of the immunodiffusion. The human semen stains can be differentiated from the animal semen stains with anti-p30 serum. Therefore, it is concluded that the demonstration of p30 in a suspected stain is the best method for human semen identification up to now.

PMID: 2480326, UI: 90077365

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Hua Hsi I Ko Ta Hsueh Hsueh Pao 1988 Jun;19(2):196-9

[Genetic studies on certain behavior traits of human beings with the twin method].

[Article in Chinese]

Hu JZ, Liu XH, Mu SH, Zhang SZ, Wu MY

PMID: 3198104, UI: 89065689

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Hua Hsi I Ko Ta Hsueh Hsueh Pao 1988 Jun;19(2):112-5

[Preparation and identification of anti-p30 serum].

[Article in Chinese]

Hou YP, Wu MY

PMID: 2461896, UI: 89065666

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Hua Hsi I Ko Ta Hsueh Hsueh Pao 1987 Dec;18(4):357-60

[Isolation and purification of p30 from human seminal plasma].

[Article in Chinese]

Hou YP, Wu MY

PMID: 2452782, UI: 88212378

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