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J Forensic Sci 1999 Nov;44(6):1273-6

Frequencies of D8S384 alleles and genotypes in European, African-American, Chinese, and Japanese populations.

Meng HY, Hou YP, Chen GD, Li YB, Wu J, Walter H, Prinz M

D8S384 is a tetranucleotide tandem repeat locus. In order to evaluate the forensic validation of D8S384, the genotype distributions and allele frequencies in ten populations from three main ethnic groups were investigated, including Germans, Slovakians, African Americans, Japanese, and Chinese (Jilin, Guangzhou, Nanning, Hailaer, Dali, and Chengdu). A total of 1011 unrelated individuals, 41 pedigrees, 30 disputed paternity trios and three personal identification cases were analyzed for D8S384 by Amp-FLP technique. Many kinds of tissues, body fluids, secreta and stains have been tested. The alleles were determined by comparison with a human allele ladder. The results showed that D8S384 typing was both precise and reliable. There were eight alleles in these populations. The genotype distributions conformed to Hardy-Weinberg equilibrium predictions. No mutation events were observed. With a maximum likelihood method, the mutation rate was indirectly estimated as 2.14 x 10(-5). The heterozygosity was 0.704 +/- 0.014 at D8S384 locus. All these results suggest that D8S384 locus is a useful marker for forensic identification and paternity analysis.

PMID: 10582368, UI: 20049081 

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J Forensic Sci 1999 Sep;44(5):1039-41

STR HUMARA locus gene and genotype frequencies in Han and Bei populations in China.

Wu MY, Zou LP, Shen B, Sun GY, Li RH, Chen GD

For the purpose of the population genetics study of the HUMARA locus, the allele, and genotype frequencies were determined in two Chinese population samples (Han-101, Bei-113) using PCR, PAGE, and silver staining. Fourteen alleles were found. The size of amplified fragments were 258 bp-315 bp. The observed heterozygosities were 0.83 in the Han population and 0.73 in the Bei population respectively. The expected heterozygosities were 0.91 in the Han population and 0.97 in the Bei population respectively. Both populations meet Hardy-Weinberg expectation, Han population x2 = 17.7206, df = 11, p > 0.05; Bei population x2 = 7.4268, df = 10, p > 0.05. The discrimination power were 0.95 in females and 0.89 in males in the Han population, 0.94 in females and 0.88 in males in the Bei population. Thus, the allelic frequency data can be used in the personal identification and parentage testing in the forensic science practice. The PCR test established in this study is robust and reproducible.

PMID: 10486954, UI: 99416422 

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J Forensic Sci 1996 Sep;41(5):859-61

Analysis of the VNTR locus DXS52 by the Amp-FLP technique.

Yun WM, Yun SG

The locus DXS52 is useful genetic marker system for forensic analysis. It consists of a variable number of tandem repeats (VNTR) and can be analyzed by the Amp-FLP technique. As accurate data about the distribution of the alleles are among the most important prerequisites for the application in forensic biology, we studied the allele distribution of DXS52 locus in a Chinese population and applied the established technique to paternity testing.

PMID: 8789847, UI: 96381834 

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